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Immunohistochemical staining of human parathyroid gland shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows moderate nuclear positivity in glandular cells.

Anti-TPI1 Antibody

HPA050924
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTPI1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TPI1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7167
  • Target name
    TPI1
  • Target description
    triosephosphate isomerase 1
  • Target synonyms
    HEL-S-49, TIM, TPI, TPID, triosephosphate isomerase, epididymis secretory protein Li 49, methylglyoxal synthase, triose-phosphate isomerase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60174
  • Protein Name
    Triosephosphate isomerase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TPI1 using anti-TPI1 antibody (A02559-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPI1 antigen affinity purified polyclonal antibody (Catalog # A02559-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPI1 at approximately 27 kDa. The expected band size for TPI1 is at 27 kDa.
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