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TPI1 Polyclonal Antibody

CAC14655
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTPI1
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Overview

  • Supplier
    Biomatik
  • Product Name
    TPI1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7167
  • Target name
    TPI1
  • Target description
    triosephosphate isomerase 1
  • Target synonyms
    epididymis secretory protein Li 49; HEL-S-49; methylglyoxal synthase; TIM; TPI; TPID; triosephosphate isomerase; triose-phosphate isomerase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60174
  • Protein Name
    Triosephosphate isomerase
  • Scientific Description
    The TPI1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HL60 whole cell lysate, Mouse brain tissue All lanes: TPI1 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31, 27, 18 kDa Observed band size: 31, 27 kDa
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Figure 1. Western blot analysis of TPI1 using anti-TPI1 antibody (A02559-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPI1 antigen affinity purified polyclonal antibody (Catalog # A02559-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPI1 at approximately 27 kDa. The expected band size for TPI1 is at 27 kDa.
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