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Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Anti-TRAF1 Antibody

HPA001852
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetTRAF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRAF1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7185
  • Target name
    TRAF1
  • Target description
    TNF receptor associated factor 1
  • Target synonyms
    EBI6; Epstein-Bar virus-induced protein 6; MGC:10353; TNF receptor-associated factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13077
  • Protein Name
    TNF receptor-associated factor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Product group Antibodies
References
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Atlas Antibodies
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Western Blot analysis of HEPG2 cells using TRAF1 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
Product group Antibodies
Cusabio
TRAF1 AntibodyCSB-PA118746
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetTRAF1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
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Figure 1. Western blot analysis of TRAF1 using anti-TRAF1 antibody (A01365). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF1 antigen affinity purified polyclonal antibody (Catalog # A01365) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF1 at approximately 46KD. The expected band size for TRAF1 is at 46KD.
Product group Antibodies
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Anti-TRAF1 Antibody Picoband(r)A01365-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetTRAF1
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