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Western Blot analysis of HEPG2 cells using TRAF1 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
Western Blot analysis of HEPG2 cells using TRAF1 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
Western Blot analysis of HEPG2 cells using TRAF1 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

TRAF1 Antibody

CSB-PA118746
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetTRAF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRAF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7185
  • Target name
    TRAF1
  • Target description
    TNF receptor associated factor 1
  • Target synonyms
    EBI6; Epstein-Bar virus-induced protein 6; MGC:10353; TNF receptor-associated factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13077
  • Protein Name
    TNF receptor-associated factor 1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRAF1 using anti-TRAF1 antibody (A01365). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF1 antigen affinity purified polyclonal antibody (Catalog # A01365) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF1 at approximately 46KD. The expected band size for TRAF1 is at 46KD.
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