More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/TRAF1 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRAF1 Polyclonal Antibody, Unconjugated (bs-1212R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRAF1 Polyclonal Antibody, Unconjugated (bs-1212R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRAF1 Polyclonal Antibody, Unconjugated (bs-1212R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining

TRAF1 Polyclonal Antibody

BS-1212R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTRAF1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    TRAF1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7185
  • Target name
    TRAF1
  • Target description
    TNF receptor associated factor 1
  • Target synonyms
    EBI6, MGC:10353, TNF receptor-associated factor 1, Epstein-Bar virus-induced protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13077
  • Protein Name
    TNF receptor-associated factor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Comparative genomic study of gastric epithelial cells co-cultured with Helicobacter pylori. Wang F et al., 2012 Dec 28, World J Gastroenterol
    Read more

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of HEPG2 cells using TRAF1 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
Product group Antibodies
Cusabio
TRAF1 AntibodyCSB-PA118746
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetTRAF1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-TRAF1EB07170
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetTRAF1
  • SizePrice
Product group Antibodies
GeneTex
TRAF1 antibody [N1C1]GTX102372
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetTRAF1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-TRAF1 (N-term) Antibody102-21655
ApplicationsWestern Blot
TargetTRAF1
  • SizePrice
Anti-TRAF1 Antibody
Product group Antibodies
Antibodies.com
Anti-TRAF1 AntibodyA80995
ApplicationsWestern Blot
  • SizePrice
Figure 1. Western blot analysis of TRAF1 using anti-TRAF1 antibody (A01365). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF1 antigen affinity purified polyclonal antibody (Catalog # A01365) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF1 at approximately 46KD. The expected band size for TRAF1 is at 46KD.
Product group Antibodies
Boster Bio
Anti-TRAF1 Antibody Picoband(r)A01365-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetTRAF1
  • SizePrice
Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Product group Antibodies
Atlas Antibodies
Anti-TRAF1 AntibodyHPA001852
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTRAF1
  • SizePrice
Product group Antibodies
Biomatik
TNF Receptor Associated Factor 1 (TRAF1) Polyclonal AntibodyCAU22343
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTRAF1
  • SizePrice
Product group Antibodies
Assay Genie
TRAF1 AntibodyCAB0150
TargetTRAF1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
TRAF1 AntibodyLS-C747454
ApplicationsWestern Blot
TargetTRAF1
  • SizePrice