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Immunohistochemistry analysis in human spleen and liver tissues using Anti-TRAF5 antibody. Corresponding TRAF5 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human spleen and liver tissues using Anti-TRAF5 antibody. Corresponding TRAF5 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human spleen and liver tissues using Anti-TRAF5 antibody. Corresponding TRAF5 RNA-seq data are presented for the same tissues.

Anti-TRAF5 Antibody

HPA008052
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRAF5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRAF5
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7188
  • Target name
    TRAF5
  • Target description
    TNF receptor associated factor 5
  • Target synonyms
    MGC:39780, RNF84, TNF receptor-associated factor 5, RING finger protein 84
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00463
  • Protein Name
    TNF receptor-associated factor 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRAF5 using anti-TRAF5 antibody (A03076-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysate,Lane 2: mouse testis tissue lysate,Lane 3: mouse thymus tissue lysate,Lane 4: human MCF-7 whole cell lysate,Lane 5: human A549 whole cell lysate,Lane 6: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF5 antigen affinity purified polyclonal antibody (Catalog # A03076-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF5 at approximately 64KD. The expected band size for TRAF5 is at 64KD.
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