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TRAF5 Antibody

LS-C763460
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetTRAF5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRAF5 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:100 - 1:400) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7188
  • Target name
    TRAF5
  • Target description
    TNF receptor associated factor 5
  • Target synonyms
    MGC:39780; RING finger protein 84; RNF84; TNF receptor-associated factor 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRAF5 using anti-TRAF5 antibody (A03076-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysate,Lane 2: mouse testis tissue lysate,Lane 3: mouse thymus tissue lysate,Lane 4: human MCF-7 whole cell lysate,Lane 5: human A549 whole cell lysate,Lane 6: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF5 antigen affinity purified polyclonal antibody (Catalog # A03076-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF5 at approximately 64KD. The expected band size for TRAF5 is at 64KD.
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