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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA024153ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA024153ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA024153ESR1HU at dilution of 1:100

TRAF5 Antibody

CSB-PA024153ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTRAF5
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRAF5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7188
  • Target name
    TRAF5
  • Target description
    TNF receptor associated factor 5
  • Target synonyms
    MGC:39780; RING finger protein 84; RNF84; TNF receptor-associated factor 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00463
  • Protein Name
    TNF receptor-associated factor 5
  • Scientific Description
    Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRAF5 using anti-TRAF5 antibody (A03076-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysate,Lane 2: mouse testis tissue lysate,Lane 3: mouse thymus tissue lysate,Lane 4: human MCF-7 whole cell lysate,Lane 5: human A549 whole cell lysate,Lane 6: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF5 antigen affinity purified polyclonal antibody (Catalog # A03076-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF5 at approximately 64KD. The expected band size for TRAF5 is at 64KD.
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