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Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.

Anti-TREM1 Antibody

HPA053612
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTREM1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TREM1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54210
  • Target name
    TREM1
  • Target description
    triggering receptor expressed on myeloid cells 1
  • Target synonyms
    CD354, TREM-1, triggering receptor expressed on myeloid cells 1, triggering receptor expressed on monocytes 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP99
  • Protein Name
    Triggering receptor expressed on myeloid cells 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TREM1 using anti-TREM1 antibody (A02135-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TREM1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREM1 antigen affinity purified polyclonal antibody (Catalog # A02135-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREM1 at approximately 26KD. The expected band size for TREM1 is at 26KD.
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