Figure 1. Western blot analysis of TREM1 using anti-TREM1 antibody (A02135-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TREM1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREM1 antigen affinity purified polyclonal antibody (Catalog # A02135-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREM1 at approximately 26KD. The expected band size for TREM1 is at 26KD.
Figure 1. Western blot analysis of TREM1 using anti-TREM1 antibody (A02135-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TREM1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREM1 antigen affinity purified polyclonal antibody (Catalog # A02135-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREM1 at approximately 26KD. The expected band size for TREM1 is at 26KD.
Anti-TREM1 Antibody Picoband(r)
A02135-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTREM1
Overview
- SupplierBoster Bio
- Product NameAnti-TREM1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID54210
- Target nameTREM1
- Target descriptiontriggering receptor expressed on myeloid cells 1
- Target synonymsCD354; TREM-1; triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1
- HostRabbit
- IsotypeIgG
- Protein IDQ9NP99
- Protein NameTriggering receptor expressed on myeloid cells 1
- Scientific DescriptionBoster Bio Anti-TREM1 Antibody Picoband® catalog # A02135-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203