Anti-TREM-1 [14F69]

Absolute Antibody

Anti-TREM-1 [14F69]

7

The original IgG2a antibody binds human TREM-1 with an affinity of 2 X 10^-10 nM as tested by suface plasmon resonance. The screening of original antibody for binding human TREM-1 was done using ELISA and flow cytometry. This antibody is capable of binding both human, cynomolgus monkey and rhesus monkey PBMCs. This antibody was reported to block the release of TNFalpha from stimulated peripheral blood mononuclear cells (PBMCs). This antibody was also able to block the ligand-induced TREM-1 activation in such BWZ/hTREM-1 reporter cell assay (US9000127).

Flow Cytometry, ELISA, Neutralisation/Blocking

ELISA; FC; Block

Research Use Only

Monoclonal

14F69

TREM1

triggering receptor expressed on myeloid cells 1

CD354; TREM-1; triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1

Human

IgG1

Triggering receptor expressed on myeloid cells 1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Human, cynomolgus monkey, rhesus monkey

The original antibody was generated by immunizing both wild type BALB/c mice and TREM-1 knock-out (KO) mice (C57BL/6 background) with either human TREM-1, cells expressing hTREM-1 (BWZ.36 cells), or a combination of both.

-20°C,2°C to 8°C

12352203