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Anti-TRIM63 Antibody

Anti-TRIM63 Antibody

A30694
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-TRIM63 Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    >95%
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to TRIM63
  • UNSPSC
    12352203

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K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MuRF1 Polyclonal Antibody(bs-2539R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
MuRF1 Polyclonal AntibodyBS-2539R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetTRIM63
  • SizePrice
Product group Antibodies
Assay Genie
TRIM63 AntibodyCAB3101
TargetTRIM63
  • SizePrice
Figure 1. Western blot analysis of MURF1/TRIM63 using anti-MURF1/TRIM63 antibody (A02016-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MURF1/TRIM63 antigen affinity purified polyclonal antibody (Catalog # A02016-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MURF1/TRIM63 at approximately 40KD. The expected band size for MURF1/TRIM63 is at 40KD.
Product group Antibodies
Boster Bio
Anti-MURF1/TRIM63 Antibody Picoband(r)A02016-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetTRIM63
  • SizePrice
Product group Antibodies
RayBiotech
Anti-TRIM63 Antibody144-65652
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetTRIM63
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-MURF1 / TRIM63 (N Term)EB05879
ApplicationsELISA, ImmunoHistoChemistry
TargetTRIM63
  • SizePrice
Product group Antibodies
GeneTex
MURF1 antibodyGTX135929
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetTRIM63
  • SizePrice
Product group Antibodies
MedChem Express
MuRF1 Antibody (YA4416, Mouse)HY-P84719
TargetTRIM63
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Product group Antibodies
LifeSpan BioSciences
MURF1 / IRF AntibodyLS-C402695
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetTRIM63
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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA168065(TRIM63 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
Product group Antibodies
Cusabio
TRIM63 AntibodyCSB-PA168065
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTRIM63
  • SizePrice