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MURF1 / IRF Antibody

LS-C402695
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTRIM63
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MURF1 / IRF Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200), WB (1:1000 - 1:5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84676
  • Target name
    TRIM63
  • Target description
    tripartite motif containing 63
  • Target synonyms
    CMH31, IRF, MURF1, MURF2, RNF28, SMRZ, E3 ubiquitin-protein ligase TRIM63, RING-type E3 ubiquitin transferase TRIM63, iris ring finger protein, muscle specific ring finger protein 2, muscle-specific RING finger protein 1, ring finger protein 28, striated muscle RING zinc finger protein, tripartite motif containing 63, E3 ubiquitin protein ligase, tripartite motif-containing protein 63
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MuRF1 Polyclonal Antibody(bs-2539R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
MuRF1 Polyclonal AntibodyBS-2539R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetTRIM63
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TRIM63 AntibodyCAB3101
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TargetTRIM63
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Product group Antibodies
Antibodies.com
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ReactivityHuman, Mouse, Rat
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Product group Antibodies
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Goat anti-MURF1 / TRIM63 (N Term) AntibodyEB05879
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TargetTRIM63
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GeneTex
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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA168065(TRIM63 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
Product group Antibodies
Cusabio
TRIM63 AntibodyCSB-PA168065
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ReactivityHuman
TargetTRIM63
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Figure 1. Western blot analysis of MURF1/TRIM63 using anti-MURF1/TRIM63 antibody (A02016-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MURF1/TRIM63 antigen affinity purified polyclonal antibody (Catalog # A02016-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MURF1/TRIM63 at approximately 40KD. The expected band size for MURF1/TRIM63 is at 40KD.
Product group Antibodies
Boster Bio
Anti-MURF1/TRIM63 Antibody Picoband(r)A02016-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Monkey
TargetTRIM63
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