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Goat anti-MURF1 / TRIM63 (N Term)

EB05879
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTRIM63
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-MURF1 / TRIM63 (N Term) Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID84676
  • Target name
    TRIM63
  • Target description
    tripartite motif containing 63
  • Target synonyms
    CMH31, IRF, MURF1, MURF2, RNF28, SMRZ, E3 ubiquitin-protein ligase TRIM63, RING-type E3 ubiquitin transferase TRIM63, iris ring finger protein, muscle specific ring finger protein 2, muscle-specific RING finger protein 1, ring finger protein 28, striated muscle RING zinc finger protein, tripartite motif containing 63, E3 ubiquitin protein ligase, tripartite motif-containing protein 63
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_115977.2. Purification: Antigen affinity purified. Names and symbols: TRIM63; MURF1; MURF-1; RNF28; ring finger protein 28; IRF; SMRZ; MURF2; FLJ32380; iris ring finger protein; muscle specific ring finger protein 2; striated muscle RING zinc finger protein; tripartite motif-containing 63; muscle specific ring finger protei
  • Reactivity Supplier
    Human, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Product group Antibodies
References
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TargetTRIM63
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Figure 1. Western blot analysis of MURF1/TRIM63 using anti-MURF1/TRIM63 antibody (A02016-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MURF1/TRIM63 antigen affinity purified polyclonal antibody (Catalog # A02016-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MURF1/TRIM63 at approximately 40KD. The expected band size for MURF1/TRIM63 is at 40KD.
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TargetTRIM63
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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA168065(TRIM63 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
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TRIM63 AntibodyCSB-PA168065
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TargetTRIM63
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