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Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Anti-TSG101 Antibody

HPA006161
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetTSG101
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TSG101
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7251
  • Target name
    TSG101
  • Target description
    tumor susceptibility 101
  • Target synonyms
    ESCRT-I complex subunit TSG101; TSG10; tumor susceptibility gene 10; tumor susceptibility gene 101; tumor susceptibility gene 101 protein; tumor susceptibility protein; VPS23
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99816
  • Protein Name
    Tumor susceptibility gene 101 protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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References
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Figure 1. Western blot analysis of TSG101 using anti-TSG101 antibody (A01233-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Colo320 whole cell lysates, Lane 3: human Sw620 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human Hek293 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 antigen affinity purified polyclonal antibody (Catalog # A01233-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG101 at approximately 44KD. The expected band size for TSG101 is at 44KD.
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