Anti-Type IV collagenase [3G11]

Absolute Antibody

Anti-Type IV collagenase [3G11]

9

The specificity of the original format of this antibody (mouse IgG1, kappa) was measured by ELISA analysis. The (131)I-labeled version of this antibody was demonstrated to be stable in vitro for almost 72 h. The blood clearance pattern of (125)I-labeled 3G11 was defined as a two-compartment model, with a 7.2 h T1/2alpha and a 345.2 h T1/2beta. A clear scintigraphic image of human lung carcinoma PG was obtained using (131)I-labeled 3G11 at 72 h, which further improved at 120 h (Dai et al., 2003; PMID: 14693044). Lidamycin (LDM) was conjugated to the Fab version of this antibody, and the resulting immunoconjugate maintained most of its immunoreactivity to both type IV collagenase and mouse hepatoma 22 cells, as shown by ELISA, and exhibited more potent cytotoxicity to hepatoma 22 cells than free LDM by MTT assay. Furthermore, when administered intravenously, the Fab-LDM conjugate was proven to be more effective against the growth of subcutaneously transplanted hepatoma 22 in mice than free LDM in two experiment settings, increasing mice survival time (Fengqiang et al., 2004; PMID: 15382678). The scFv version of this antibody was constructed and used in various configurations for IF and in vivo imaging and therapeutic applications (Zhong et al., 2010; PMID: 20303650) (Kong et al., 2015; PMID: 25824464).

ImmunoFluorescence, ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

3G11

MMP2

matrix metallopeptidase 2

CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1, 72 kDa type IV collagenase, collagenase type IV-A, matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), matrix metalloproteinase-2, matrix metalloproteinase-II, neutrophil gelatinase

Mouse

IgG1

72 kDa type IV collagenase

Human

-20°C,2°C to 8°C

41116161