More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-Type IV collagenase [3G11]

Anti-Type IV collagenase [3G11]

AB00421-1.1-BT
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetMMP2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Type IV collagenase [3G11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody binds to human type IV collagenase, a metalloproteinase involved in degrading matrix proteins and hence contributing largely to metastasis in various types of cancer.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3G11
  • Gene ID4313
  • Target name
    MMP2
  • Target description
    matrix metallopeptidase 2
  • Target synonyms
    72 kDa type IV collagenase; CLG4; CLG4A; collagenase type IV-A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase-2; matrix metalloproteinase-II; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08253
  • Protein Name
    72 kDa type IV collagenase
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated bs-0412R at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
References
Bioss Antibodies
MMP2 Polyclonal AntibodyBS-0412R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMMP2
  • SizePrice
Product group Antibodies
Assay Genie
MMP2 AntibodyCAB6247
TargetMMP2
  • SizePrice
Product group Antibodies
Biosynth
MMP2 antibody10R-M146A
  • SizePrice
Figure 1. Western blot analysis of MMP2 using anti-MMP2 antibody (A00286-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP2 antigen affinity purified polyclonal antibody (Catalog # A00286-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP2 at approximately 72KD. The expected band size for MMP2 is at 72KD.
Product group Antibodies
Boster Bio
Anti-MMP2 Antibody Picoband(r)A00286-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetMMP2
  • SizePrice
Anti-MMP-2 Antibody
Product group Antibodies
Antibodies.com
Anti-MMP-2 AntibodyA97407
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MMP2 Antibody144-06247
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetMMP2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MMP2 AntibodyLS-C831544
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetMMP2
  • SizePrice
Product group Antibodies
Biomatik
Mmp2 Polyclonal AntibodyCAC09087
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetMMP2
  • SizePrice
Immunohistochemical staining of human urinary bladder shows weak to moderate cytoplasmic positivity in urothelial cells.
Product group Antibodies
Atlas Antibodies
Anti-MMP2 AntibodyHPA001939
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetMMP2
  • SizePrice
Product group Antibodies
References
GeneTex
MMP2 antibodyGTX104577
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMMP2
  • SizePrice