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Western Blot analysis of HT29 cells using MMP-2 Polyclonal Antibody
Western Blot analysis of HT29 cells using MMP-2 Polyclonal Antibody
Western Blot analysis of HT29 cells using MMP-2 Polyclonal Antibody

MMP2 Antibody

CSB-PA003258
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetMMP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    MMP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4313
  • Target name
    MMP2
  • Target description
    matrix metallopeptidase 2
  • Target synonyms
    CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1, 72 kDa type IV collagenase, collagenase type IV-A, matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), matrix metalloproteinase-2, matrix metalloproteinase-II, neutrophil gelatinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08253
  • Protein Name
    72 kDa type IV collagenase
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MMP2 using anti-MMP2 antibody (A00286-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP2 antigen affinity purified polyclonal antibody (Catalog # A00286-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP2 at approximately 72KD. The expected band size for MMP2 is at 72KD.
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