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Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated bs-0412R at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated bs-0412R at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated bs-0412R at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

MMP2 Polyclonal Antibody

BS-0412R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMMP2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MMP2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4313
  • Target name
    MMP2
  • Target description
    matrix metallopeptidase 2
  • Target synonyms
    72 kDa type IV collagenase; CLG4; CLG4A; collagenase type IV-A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase-2; matrix metalloproteinase-II; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08253
  • Protein Name
    72 kDa type IV collagenase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • MAY, a novel tubulin inhibitor, induces cell apoptosis in A549 and A549/Taxol cells and inhibits epithelial-mesenchymal transition in A549/Taxol cells. Du J et al., 2020 May 25, Chem Biol Interact
    Read more
  • The role of miR-21/RECK in the inhibition of osteosarcoma by curcumin. Zhou L et al., 2020 Jun, Mol Cell Probes
    Read more
  • The effect of a new wound dressing on wound healing: Biochemical and histopathological evaluation. Tort S et al., 2020 Feb, Burns
    Read more
  • Effects of uterus derived mesenchymal stem cells and their exosomes on ashermans syndrome. Saribas GS et al., 2020 Jan, Acta Histochem
    Read more
  • Ligustilide Inhibited Rat Vascular Smooth Muscle Cells Migration via c-Myc/MMP2 and ROCK/JNK Signaling Pathway. Luo Z et al., 2019 Dec, J Food Sci
    Read more
  • MiR-203a-3p inhibits retinal angiogenesis and alleviates proliferative diabetic retinopathy in oxygen-induced retinopathy (OIR) rat model via targeting VEGFA and HIF-1alpha. Han N et al., 2020 Jan, Clin Exp Pharmacol Physiol
    Read more
  • Ginkgo biloba extract may alleviate viral myocarditis by suppression of S100A4 and MMP-3. Wang W et al., 2019 Dec, J Med Virol
    Read more
  • miR-363-3p inhibits migration, invasion, and epithelial-mesenchymal transition by targeting NEDD9 and SOX4 in non-small-cell lung cancer. Chang J et al., 2019 Jul 22, J Cell Physiol
    Read more
  • MicroRNA-7 as a potential therapeutic target for aberrant NF-kappaB-driven distant metastasis of gastric cancer. Ye T et al., 2019 Feb 6, J Exp Clin Cancer Res
    Read more
  • The long noncoding RNA HOTTIP promotes breast cancer cell migration, invasiveness, and epithelial-mesenchymal transition via the Wnt-beta-catenin signaling pathway. Han S et al., 2019 Oct, Biochem Cell Biol
    Read more

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Figure 1. Western blot analysis of MMP2 using anti-MMP2 antibody (A00286-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP2 antigen affinity purified polyclonal antibody (Catalog # A00286-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP2 at approximately 72KD. The expected band size for MMP2 is at 72KD.
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