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Immunohistochemical staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Anti-UBE2S Antibody

HPA057150
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetUBE2S
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UBE2S
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27338
  • Target name
    UBE2S
  • Target description
    ubiquitin conjugating enzyme E2 S
  • Target synonyms
    E2-EPF, E2EPF, EPF5, ubiquitin-conjugating enzyme E2 S, E2 ubiquitin-conjugating enzyme S, E2-EPF5, ubiquitin carrier protein S, ubiquitin conjugating enzyme E2S, ubiquitin-conjugating enzyme E2-24 kDa, ubiquitin-conjugating enzyme E2-EPF5, ubiquitin-protein ligase S
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16763
  • Protein Name
    Ubiquitin-conjugating enzyme E2 S
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of UBE2S using anti-UBE2S antibody (A03045-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2S antigen affinity purified polyclonal antibody (Catalog # A03045-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE2S at approximately 24 kDa. The expected band size for UBE2S is at 24 kDa.
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