
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with E2EPF Polyclonal Antibody, Unconjugated (bs-2305R) at 1:200 overnight at 4°C, DAB staining.
E2EPF Polyclonal Antibody
BS-2305R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetUBE2S
Overview
- SupplierBioss
- Product NameE2EPF Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID27338
- Target nameUBE2S
- Target descriptionubiquitin conjugating enzyme E2 S
- Target synonymsE2-EPF, E2EPF, EPF5, ubiquitin-conjugating enzyme E2 S, E2 ubiquitin-conjugating enzyme S, E2-EPF5, ubiquitin carrier protein S, ubiquitin conjugating enzyme E2S, ubiquitin-conjugating enzyme E2-24 kDa, ubiquitin-conjugating enzyme E2-EPF5, ubiquitin-protein ligase S
- HostRabbit
- IsotypeIgG
- ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203





![Wild-type (WT) and UBE2S knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with UBE2S antibody [N1C2] (GTX115862) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115862/GTX115862_40296_20180105_WB_KO_watermark_w_23060519_223.webp)
