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ProductsBack/Anti-Ubiquitin-like protein ATG12 ATG12 Antibody
Western blot analysis of ATG12 in human brain tissue lysate with ATG12 antibody at (A) 0.5
Western blot analysis of ATG12 in human brain tissue lysate with ATG12 antibody at (A) 0.5
Western blot analysis of ATG12 in human brain tissue lysate with ATG12 antibody at (A) 0.5

Anti-Ubiquitin-like protein ATG12 ATG12 Antibody

A00820
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATG12
0.1 mg
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Ubiquitin-like protein ATG12 ATG12 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID9140
  • Target name
    ATG12
  • Target description
    autophagy related 12
  • Target synonyms
    APG12, APG12L, FBR93, HAPG12, ubiquitin-like protein ATG12, APG12 autophagy 12-like, ATG12 autophagy related 12 homolog, Apg12 (autophagy, yeast) homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94817
  • Protein Name
    Ubiquitin-like protein ATG12
  • Scientific Description
    Boster Bio Anti-Ubiquitin-like protein ATG12 ATG12 Antibody (Catalog # A00820). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of ATG12 using anti-ATG12 antibody (A00820-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human COLO 320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG12 antigen affinity purified polyclonal antibody (Catalog # A00820-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG12 at approximately 48 kDa. The expected band size for ATG12 is at 15 kDa.
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