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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Anti-UBR2 Antibody

HPA027869
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetUBR2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UBR2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23304
  • Target name
    UBR2
  • Target description
    ubiquitin protein ligase E3 component n-recognin 2
  • Target synonyms
    C6orf133, bA49A4.1, dJ242G1.1, dJ392M17.3, E3 ubiquitin-protein ligase UBR2, RING-type E3 ubiquitin transferase UBR2, ubiquitin-protein ligase E3-alpha-2, ubiquitin-protein ligase E3-alpha-II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IWV8
  • Protein Name
    E3 ubiquitin-protein ligase UBR2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of UBR2 using anti-UBR2 antibody (A05812-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse RAW264.7 tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBR2 antigen affinity purified polyclonal antibody (Catalog # A05812-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBR2 at approximately 201KD. The expected band size for UBR2 is at 201KD.
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