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Immunohistochemistry analysis in human kidney and lymph node tissues using HPA054065 antibody. Corresponding UGT1A6 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human kidney and lymph node tissues using HPA054065 antibody. Corresponding UGT1A6 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human kidney and lymph node tissues using HPA054065 antibody. Corresponding UGT1A6 RNA-seq data are presented for the same tissues.

Anti-UGT1A6 Antibody

HPA054065
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetUGT1A6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UGT1A6
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54578
  • Target name
    UGT1A6
  • Target description
    UDP glucuronosyltransferase family 1 member A6
  • Target synonyms
    Bilirubin-specific UDPGT isozyme 1; GNT1; HLUGP; HLUGP1; hUG-BR1; phenol-metabolizing UDP-glucuronosyltransferase; UDP glucuronosyltransferase 1 family, polypeptide A6; UDP glycosyltransferase 1 family, polypeptide A6; UDP-glucuronosyltransferase 1 family polypeptide A6s; UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1-3; UDP-glucuronosyltransferase 1-5; UDP-glucuronosyltransferase 1-6; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1; UDP-glucuronosyltransferase 1A3; UDP-glucuronosyltransferase 1A5; UDP-glucuronosyltransferase 1A6; UDP-glucuronosyltransferase 1-C; UDP-glucuronosyltransferase 1-E; UDP-glucuronosyltransferase 1-F; UDPGT; UDPGT 1-6; UGT1; UGT1.1; UGT1.3; UGT1.5; UGT1.6; UGT1-01; UGT1-03; UGT1-05; UGT1-06; UGT1A; UGT-1A; UGT1A1; UGT1A3; UGT1A5; UGT1A6S; UGT1C; UGT-1C; UGT1E; UGT-1E; UGT1F; UGT-1F
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19224
  • Protein Name
    UDP-glucuronosyltransferase 1-6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA025579LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of UGT1A6 using anti-UGT1A6 antibody (A02239-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UGT1A6 antigen affinity purified polyclonal antibody (Catalog # A02239-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UGT1A6 at approximately 55-61KD. The expected band size for UGT1A6 is at 61KD.
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