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UGT1A6 Antibody

LS-C496745
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetUGT1A6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    UGT1A6 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 29kDa/50kDa/60kDa, while the observed MW by Western blot was 75kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB (1:500 - 1:1000) The predicted MW is 29kDa/50kDa/60kDa, while the observed MW by Western blot was 75kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54578
  • Target name
    UGT1A6
  • Target description
    UDP glucuronosyltransferase family 1 member A6
  • Target synonyms
    GNT1, HLUGP, HLUGP1, UDPGT, UDPGT 1-6, UGT-1A, UGT-1C, UGT-1E, UGT-1F, UGT1, UGT1-01, UGT1-03, UGT1-05, UGT1-06, UGT1.1, UGT1.3, UGT1.5, UGT1.6, UGT1A, UGT1A1, UGT1A3, UGT1A5, UGT1A6S, UGT1C, UGT1E, UGT1F, hUG-BR1, UDP-glucuronosyltransferase 1A6, Bilirubin-specific UDPGT isozyme 1, UDP glucuronosyltransferase 1 family, polypeptide A6, UDP glycosyltransferase 1 family, polypeptide A6, UDP-glucuronosyltransferase 1 family polypeptide A6s, UDP-glucuronosyltransferase 1-1, UDP-glucuronosyltransferase 1-3, UDP-glucuronosyltransferase 1-5, UDP-glucuronosyltransferase 1-6, UDP-glucuronosyltransferase 1-A, UDP-glucuronosyltransferase 1-C, UDP-glucuronosyltransferase 1-E, UDP-glucuronosyltransferase 1-F, UDP-glucuronosyltransferase 1A1, UDP-glucuronosyltransferase 1A3, UDP-glucuronosyltransferase 1A5, phenol-metabolizing UDP-glucuronosyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UGT1A6 using anti-UGT1A6 antibody (A02239-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UGT1A6 antigen affinity purified polyclonal antibody (Catalog # A02239-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UGT1A6 at approximately 55-61KD. The expected band size for UGT1A6 is at 61KD.
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