
Figure 1. Western blot analysis of USP25 using anti-USP25 antibody (A06182-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey liver tissue lysates, Lane 4: monkey kidney tissue lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: human PC-12 whole cell lysates, Lane 12: mouse lung tissue lysates, Lane 13: mouse RAW264.7 whole cell lysates, Lane 14: mouse NIH-3T3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP25 antigen affinity purified polyclonal antibody (Catalog # A06182-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP25 at approximately 125KD. The expected band size for USP25 is at 125KD.
Anti-USP25 Antibody Picoband(r)
A06182-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetUSP25
Overview
- SupplierBoster Bio
- Product NameAnti-USP25 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID29761
- Target nameUSP25
- Target descriptionubiquitin specific peptidase 25
- Target synonymsdeubiquitinating enzyme 25; ubiquitin carboxyl-terminal hydrolase 25; ubiquitin thioesterase 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25; USP on chromosome 21; USP21
- HostRabbit
- IsotypeIgG
- Protein IDQ9UHP3
- Protein NameUbiquitin carboxyl-terminal hydrolase 25
- Scientific DescriptionBoster Bio Anti-USP25 Antibody Picoband® catalog # A06182-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203