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WB analysis of mouse brain tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX17216 USP25 antibody. Working concentration : 1 microg/ml
WB analysis of mouse brain tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX17216 USP25 antibody. Working concentration : 1 microg/ml
WB analysis of mouse brain tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX17216 USP25 antibody. Working concentration : 1 microg/ml

USP25 antibody

GTX17216
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetUSP25
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Overview

  • Supplier
    GeneTex
  • Product Name
    USP25 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID29761
  • Target name
    USP25
  • Target description
    ubiquitin specific peptidase 25
  • Target synonyms
    deubiquitinating enzyme 25; ubiquitin carboxyl-terminal hydrolase 25; ubiquitin thioesterase 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25; USP on chromosome 21; USP21
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHP3
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 25
  • Scientific Description
    Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of USP25 using anti-USP25 antibody (A06182-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey liver tissue lysates, Lane 4: monkey kidney tissue lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: human PC-12 whole cell lysates, Lane 12: mouse lung tissue lysates, Lane 13: mouse RAW264.7 whole cell lysates, Lane 14: mouse NIH-3T3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP25 antigen affinity purified polyclonal antibody (Catalog # A06182-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP25 at approximately 125KD. The expected band size for USP25 is at 125KD.
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