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USP25 Antibody

CAB12588
Assay Genie
TargetUSP25
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    USP25 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID29761
  • Target name
    USP25
  • Target description
    ubiquitin specific peptidase 25
  • Target synonyms
    deubiquitinating enzyme 25; ubiquitin carboxyl-terminal hydrolase 25; ubiquitin thioesterase 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25; USP on chromosome 21; USP21
  • Host
    Rabbit
  • Protein IDQ9UHP3
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 25
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of USP25 using anti-USP25 antibody (A06182-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey liver tissue lysates, Lane 4: monkey kidney tissue lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: human PC-12 whole cell lysates, Lane 12: mouse lung tissue lysates, Lane 13: mouse RAW264.7 whole cell lysates, Lane 14: mouse NIH-3T3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP25 antigen affinity purified polyclonal antibody (Catalog # A06182-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP25 at approximately 125KD. The expected band size for USP25 is at 125KD.
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