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Immunohistochemical staining of human prostate shows moderate to strong membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows moderate to strong membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows moderate to strong membranous positivity in glandular cells.

Anti-UTRN Antibody

HPA018894
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetUTRN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UTRN Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7402
  • Target name
    UTRN
  • Target description
    utrophin
  • Target synonyms
    DMDL, DRP, DRP1, utrophin, dystrophin-related protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46939
  • Protein Name
    Utrophin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Utrophin/UTRN using anti-Utrophin/UTRN antibody (A02194-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Sw620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Utrophin/UTRN antigen affinity purified polyclonal antibody (Catalog # A02194-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Utrophin/UTRN at approximately 394KD. The expected band size for Utrophin/UTRN is at 394KD.
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