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Utrophin Polyclonal Antibody

BS-11394R
Bioss
ApplicationsFlow Cytometry, ELISA
Product group Antibodies
TargetUTRN
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Overview

  • Supplier
    Bioss
  • Product Name
    Utrophin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ELISA
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7402
  • Target name
    UTRN
  • Target description
    utrophin
  • Target synonyms
    DMDL, DRP, DRP1, utrophin, dystrophin-related protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Utrophin/UTRN using anti-Utrophin/UTRN antibody (A02194-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Sw620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Utrophin/UTRN antigen affinity purified polyclonal antibody (Catalog # A02194-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Utrophin/UTRN at approximately 394KD. The expected band size for Utrophin/UTRN is at 394KD.
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