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Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.

Anti-VCAN Antibody

HPA004726
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetVCAN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-VCAN
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1462
  • Target name
    VCAN
  • Target description
    versican
  • Target synonyms
    chondroitin sulfate proteoglycan core protein 2; CSPG2; ERVR; GHAP; glial hyaluronate-binding protein; large fibroblast proteoglycan; PG-M; versican core protein; versican proteoglycan; WGN; WGN1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13611
  • Protein Name
    Versican core protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Vitronectin using anti-Vitronectin antibody (PB9453). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: HEPA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitronectin antigen affinity purified polyclonal antibody (Catalog # PB9453) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitronectin at approximately 373 kDa. The expected band size for Vitronectin is at 373 kDa.
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