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Versican (VCAN) Polyclonal Antibody

CAU25246
Biomatik
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetVCAN
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Overview

  • Supplier
    Biomatik
  • Product Name
    Versican (VCAN) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1462
  • Target name
    VCAN
  • Target description
    versican
  • Target synonyms
    CSPG2, ERVR, GHAP, PG-M, WGN, WGN1, versican core protein, chondroitin sulfate proteoglycan core protein 2, glial hyaluronate-binding protein, large fibroblast proteoglycan, versican proteoglycan
  • Host
    Rabbit
  • Protein IDP13611
  • Protein Name
    Versican core protein
  • Scientific Description
    The Versican (VCAN) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Vitronectin using anti-Vitronectin antibody (PB9453). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: HEPA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitronectin antigen affinity purified polyclonal antibody (Catalog # PB9453) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitronectin at approximately 373 kDa. The expected band size for Vitronectin is at 373 kDa.
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