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Western Blot analysis of mouse-brain cells using Versican Polyclonal Antibody
Western Blot analysis of mouse-brain cells using Versican Polyclonal Antibody
Western Blot analysis of mouse-brain cells using Versican Polyclonal Antibody

VCAN Antibody

CSB-PA007374
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetVCAN
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Overview

  • Supplier
    Cusabio
  • Product Name
    VCAN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1462
  • Target name
    VCAN
  • Target description
    versican
  • Target synonyms
    chondroitin sulfate proteoglycan core protein 2; CSPG2; ERVR; GHAP; glial hyaluronate-binding protein; large fibroblast proteoglycan; PG-M; versican core protein; versican proteoglycan; WGN; WGN1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13611
  • Protein Name
    Versican core protein
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Versican Polyclonal Antibody (bs-2533R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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Figure 1. Western blot analysis of Vitronectin using anti-Vitronectin antibody (PB9453). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: HEPA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitronectin antigen affinity purified polyclonal antibody (Catalog # PB9453) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitronectin at approximately 373 kDa. The expected band size for Vitronectin is at 373 kDa.
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