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Anti-Vimentin [RV202]

AB03356-1.1
Absolute Antibody
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Chicken, Goat, Hamster, Human, Monkey, Mouse, Porcine, Rat, Xenopus, Zebra Fish
TargetVIM
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Vimentin [RV202]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody detected vimentin by western blot analysis. This antibody was used for vimentin detection on rabbit embryo, dermatome, myotome (adjacent abdominal wall) and the genital ridge (comprising mesonephric blastema and mesonephric duct) by immunohistochemistry (Viebahn et al., 1988; PMID: 2460241). The original antibody detected vimentin on HeLa cells transfected with pRSVDes by immunohistochemistry (Raats et al., 1990; PMID: 1699950).
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RV202
  • Gene ID7431
  • Target name
    VIM
  • Target description
    vimentin
  • Target synonyms
    vimentin, epididymis secretory sperm binding protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08670
  • Protein Name
    Vimentin
  • Reactivity
    Canine, Chicken, Goat, Hamster, Human, Monkey, Mouse, Porcine, Rat, Xenopus, Zebra Fish
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Vimentin using anti-Vimentin antibody (PB9359). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human MCF-7 whole cell lysates, Lane 9: rat ovary tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (Catalog # PB9359) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54 kDa. The expected band size for Vimentin is at 54 kDa.
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