More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-Vimentin [RV203]

Anti-Vimentin [RV203]

AB03357-10.3
Absolute Antibody
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Goat, Hamster, Human, Porcine, Rat
TargetVIM
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Vimentin [RV203]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody detected vimentin on cytoskeletal extracts of bovine lens, BHK-21 and HeLa cells by western blot analysis. The antibody detected vimentin on connective tissue fibroblasts of hamster tongue, polygonal BHK-21/C13 cells and elongated BHK-21 cells by immunohistochemistry (Schaart et al., 1991; PMID: 2065865).
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RV203
  • Gene ID7431
  • Target name
    VIM
  • Target description
    vimentin
  • Target synonyms
    vimentin, epididymis secretory sperm binding protein
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP08670
  • Protein Name
    Vimentin
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Canine, Goat, Hamster, Human, Porcine, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Immunohistochemical staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.
Product group Antibodies
Atlas Antibodies
Anti-VIM AntibodyAMAB90516
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetVIM
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Vimentin [1433]AB01640-1.9-C
ApplicationsImmunoFluorescence
ReactivityCanine, Human, Rat
TargetVIM
  • SizePrice
Product group Antibodies
MedChem Express
Vimentin Antibody (YA011, Rabbit)HY-P80371
TargetVIM
  • SizePrice
Product group Antibodies
References
Everest Biotech
Goat anti-VimentinEB11207
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetVIM
  • SizePrice
Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain tissue, diluted at 1:2000.
Product group Antibodies
Cusabio
VIM Monoclonal AntibodyCSB-MA000319
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetVIM
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
Vimentin Antibody (Preservative Free)LS-C343535
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetVIM
  • SizePrice
Product group Antibodies
Nordic-MUbio
Mouse anti VimentinMUB1902P-CE/IVD
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityChicken, Human, Mammals, Rat, Sheep
TargetVIM
  • SizePrice
Product group Antibodies
Biomatik
Vim Polyclonal AntibodyCAC07060
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetVIM
  • SizePrice
Figure 1. Western blot analysis of Vimentin using anti-Vimentin antibody (PB9359). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human MCF-7 whole cell lysates, Lane 9: rat ovary tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (Catalog # PB9359) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54 kDa. The expected band size for Vimentin is at 54 kDa.
Product group Antibodies
Boster Bio
Anti-Vimentin Antibody Picoband(r)PB9359-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetVIM
  • SizePrice
Vimentin antibody detects Vimentin protein by immunohistochemical analysis. Sample: Paraffin-embedded dog pancreas. Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
GeneTex
Vimentin antibodyGTX100619
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityCanine, Feline, Human, Mouse, Rat
TargetVIM
  • SizePrice