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Immunohistochemical staining of human lung shows strong cytoplasmic positivity in pneumocytes.
Immunohistochemical staining of human lung shows strong cytoplasmic positivity in pneumocytes.
Immunohistochemical staining of human lung shows strong cytoplasmic positivity in pneumocytes.

Anti-VIPR2 Antibody

HPA062707
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetVIPR2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-VIPR2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7434
  • Target name
    VIPR2
  • Target description
    vasoactive intestinal peptide receptor 2
  • Target synonyms
    C16DUPq36.3, DUP7q36.3, PACAP-R-3, PACAP-R3, VIP-R-2, VPAC2, VPAC2R, VPCAP2R, vasoactive intestinal polypeptide receptor 2, PACAP type III receptor, VIP and PACAP receptor 2, VPAC2 receptor, helodermin-preferring VIP receptor, pituitary adenylate cyclase-activating polypeptide type III receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41587
  • Protein Name
    Vasoactive intestinal polypeptide receptor 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of VPAC2/VIPR2 using anti-VPAC2/VIPR2 antibody (A03768-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPAC2/VIPR2 antigen affinity purified polyclonal antibody (Catalog # A03768-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPAC2/VIPR2 at approximately 49 kDa. The expected band size for VPAC2/VIPR2 is at 49 kDa.
Product group Antibodies
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ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey
TargetVIPR2
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Product group Antibodies
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Protein IDP41587
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Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VPAC2 antibody [HL3625] (GTX641600) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
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TargetVIPR2
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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: VIPR2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 47 kDa Observed band size: 50 kDa
Product group Antibodies
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TargetVIPR2
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