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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: VIPR2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 47 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: VIPR2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 47 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: VIPR2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 47 kDa Observed band size: 50 kDa

VIPR2 Antibody

CSB-PA025860LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetVIPR2
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Overview

  • Supplier
    Cusabio
  • Product Name
    VIPR2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7434
  • Target name
    VIPR2
  • Target description
    vasoactive intestinal peptide receptor 2
  • Target synonyms
    C16DUPq36.3, DUP7q36.3, PACAP-R-3, PACAP-R3, VIP-R-2, VPAC2, VPAC2R, VPCAP2R, vasoactive intestinal polypeptide receptor 2, PACAP type III receptor, VIP and PACAP receptor 2, VPAC2 receptor, helodermin-preferring VIP receptor, pituitary adenylate cyclase-activating polypeptide type III receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41587
  • Protein Name
    Vasoactive intestinal polypeptide receptor 2
  • Scientific Description
    This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of VPAC2/VIPR2 using anti-VPAC2/VIPR2 antibody (A03768-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPAC2/VIPR2 antigen affinity purified polyclonal antibody (Catalog # A03768-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPAC2/VIPR2 at approximately 49 kDa. The expected band size for VPAC2/VIPR2 is at 49 kDa.
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