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Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Anti-VPS4B Antibody

HPA057649
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetVPS4B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-VPS4B Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9525
  • Target name
    VPS4B
  • Target description
    vacuolar protein sorting 4 homolog B
  • Target synonyms
    MIG1, SKD1, SKD1B, VPS4-2, vacuolar protein sorting-associated protein 4B, cell migration-inducing 1, cell migration-inducing gene 1 protein, suppressor of K(+) transport growth defect 1, suppressor of K+ transport defect 1, vacuolar protein sorting 4B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75351
  • Protein Name
    Vacuolar protein sorting-associated protein 4B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of VPS4B/MIG1 using anti-VPS4B/MIG1 antibody (A03403-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SKOV-3 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS4B/MIG1 antigen affinity purified polyclonal antibody (Catalog # A03403-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS4B/MIG1 at approximately 50KD. The expected band size for VPS4B/MIG1 is at 50KD.
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