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Immunohistochemical staining of human retina shows strong nuclear positivity in inner nuclear layer.
Immunohistochemical staining of human retina shows strong nuclear positivity in inner nuclear layer.
Immunohistochemical staining of human retina shows strong nuclear positivity in inner nuclear layer.

Anti-VSX2 Antibody

HPA003436
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetVSX2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-VSX2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID338917
  • Target name
    VSX2
  • Target description
    visual system homeobox 2
  • Target synonyms
    CHX10, HOX10, MCOP2, MCOPCB3, RET1, visual system homeobox 2, ceh-10 homeo domain containing homolog, ceh-10 homeodomain-containing homolog, homeobox protein CHX10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP58304
  • Protein Name
    Visual system homeobox 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CHX10 using anti-CHX10 antibody (A05180). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse eye ball tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHX10 antigen affinity purified polyclonal antibody (Catalog # A05180) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHX10 at approximately 43KD. The expected band size for CHX10 is at 39KD.
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CHX10 antibody detects CHX10 protein by immunohistochemical analysis. Samples: Paraffin-embedded mouse retina. Green: CHX10 protein stained by CHX10 antibody (GTX114143) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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