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CHX10 antibody detects CHX10 protein by immunohistochemical analysis. Samples: Paraffin-embedded mouse retina. Green: CHX10 protein stained by CHX10 antibody (GTX114143) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CHX10 antibody detects CHX10 protein by immunohistochemical analysis. Samples: Paraffin-embedded mouse retina. Green: CHX10 protein stained by CHX10 antibody (GTX114143) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CHX10 antibody detects CHX10 protein by immunohistochemical analysis. Samples: Paraffin-embedded mouse retina. Green: CHX10 protein stained by CHX10 antibody (GTX114143) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CHX10 antibody

GTX114143
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Human, Mouse
TargetVSX2
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Overview

  • Supplier
    GeneTex
  • Product Name
    CHX10 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.85 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID338917
  • Target name
    VSX2
  • Target description
    visual system homeobox 2
  • Target synonyms
    CHX10, HOX10, MCOP2, MCOPCB3, RET1, visual system homeobox 2, ceh-10 homeo domain containing homolog, ceh-10 homeodomain-containing homolog, homeobox protein CHX10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP58304
  • Protein Name
    Visual system homeobox 2
  • Scientific Description
    This gene encodes a homeobox protein originally described as a retina-specific transcription factor. Mutations in this gene are associated with microphthalmia, cataracts and iris abnormalities. [provided by RefSeq]
  • Reactivity
    Chicken, Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yamagata M, Yan W, Sanes JR. A cell atlas of the chick retina based on single-cell transcriptomics. Elife. 2021,10. doi: 10.7554/eLife.63907
    Read this paper

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Figure 1. Western blot analysis of CHX10 using anti-CHX10 antibody (A05180). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse eye ball tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHX10 antigen affinity purified polyclonal antibody (Catalog # A05180) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHX10 at approximately 43KD. The expected band size for CHX10 is at 39KD.
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