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CHX10 Antibody (YA2790, Rabbit)

HY-P83045
MedChem Express
TargetVSX2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CHX10 Antibody (YA2790, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID338917
  • Target name
    VSX2
  • Target description
    visual system homeobox 2
  • Target synonyms
    CHX10, HOX10, MCOP2, MCOPCB3, RET1, visual system homeobox 2, ceh-10 homeo domain containing homolog, ceh-10 homeodomain-containing homolog, homeobox protein CHX10
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CHX10 Antibody (YA2790) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2790), targeting CHX10, with a predicted molecular weight of 39 kDa (observed band size: 50 kDa). CHX10 Antibody (YA2790) can be used for WB, FC experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CHX10 using anti-CHX10 antibody (A05180). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse eye ball tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHX10 antigen affinity purified polyclonal antibody (Catalog # A05180) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHX10 at approximately 43KD. The expected band size for CHX10 is at 39KD.
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