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Immunohistochemical staining of human endometrium shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human endometrium shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human endometrium shows strong cytoplasmic positivity in glandular cells.

Anti-WDR1 Antibody

HPA036434
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetWDR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WDR1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9948
  • Target name
    WDR1
  • Target description
    WD repeat domain 1
  • Target synonyms
    actin-interacting protein 1; AIP1; epididymis secretory protein Li 52; HEL-S-52; NORI-1; PFITS; WD repeat-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75083
  • Protein Name
    WD repeat-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WDR1 using anti-WDR1 antibody (PB9962). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR1 antigen affinity purified polyclonal antibody (Catalog # PB9962) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR1 at approximately 66 kDa. The expected band size for WDR1 is at 66 kDa.
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