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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cell junctions.
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cell junctions.
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cell junctions.

Anti-WDR1 Antibody

HPA070293
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetWDR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WDR1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9948
  • Target name
    WDR1
  • Target description
    WD repeat domain 1
  • Target synonyms
    actin-interacting protein 1; AIP1; epididymis secretory protein Li 52; HEL-S-52; NORI-1; PFITS; WD repeat-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75083
  • Protein Name
    WD repeat-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WDR1 using anti-WDR1 antibody (PB9962). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR1 antigen affinity purified polyclonal antibody (Catalog # PB9962) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR1 at approximately 66 kDa. The expected band size for WDR1 is at 66 kDa.
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