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Western blot analysis in control (vector only transfected HEK293T lysate) and WEE1 over-expression lysate (Transient overexpression lysate of WEE1 homolog (S. pombe) (WEE1), transcript variant 1, LY418682).
Western blot analysis in control (vector only transfected HEK293T lysate) and WEE1 over-expression lysate (Transient overexpression lysate of WEE1 homolog (S. pombe) (WEE1), transcript variant 1, LY418682).
Western blot analysis in control (vector only transfected HEK293T lysate) and WEE1 over-expression lysate (Transient overexpression lysate of WEE1 homolog (S. pombe) (WEE1), transcript variant 1, LY418682).

Anti-WEE1 Antibody

HPA068845
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetWEE1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WEE1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7465
  • Target name
    WEE1
  • Target description
    WEE1 G2 checkpoint kinase
  • Target synonyms
    WEE1A, WEE1hu, wee1-like protein kinase, WEE1 homolog, WEE1+ homolog, protein kinase, wee1A kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30291
  • Protein Name
    Wee1-like protein kinase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of WEE1 using anti-WEE1 antibody (A01319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WEE1 antigen affinity purified polyclonal antibody (Catalog # A01319-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WEE1 at approximately 72KD. The expected band size for WEE1 is at 72KD.
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