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Western Blot analysis of 293 cells using Phospho-Wee1 (S642) Polyclonal Antibody
Western Blot analysis of 293 cells using Phospho-Wee1 (S642) Polyclonal Antibody
Western Blot analysis of 293 cells using Phospho-Wee1 (S642) Polyclonal Antibody

Phospho-WEE1 (S642) Antibody

CSB-PA060083
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetWEE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-WEE1 (S642) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7465
  • Target name
    WEE1
  • Target description
    WEE1 G2 checkpoint kinase
  • Target synonyms
    WEE1A, WEE1hu, wee1-like protein kinase, WEE1 homolog, WEE1+ homolog, protein kinase, wee1A kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30291
  • Protein Name
    Wee1-like protein kinase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (GTX111392) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of WEE1 using anti-WEE1 antibody (A01319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WEE1 antigen affinity purified polyclonal antibody (Catalog # A01319-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WEE1 at approximately 72KD. The expected band size for WEE1 is at 72KD.
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