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ProductsBack/WEE1 antibody [N3C2], Internal

WEE1 antibody [N3C2], Internal

GTX111392
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetWEE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    WEE1 antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.83 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7465
  • Target name
    WEE1
  • Target description
    WEE1 G2 checkpoint kinase
  • Target synonyms
    WEE1A, WEE1hu, wee1-like protein kinase, WEE1 homolog, WEE1+ homolog, protein kinase, wee1A kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30291
  • Protein Name
    Wee1-like protein kinase
  • Scientific Description
    This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Chen CP, Yeh CN, Pan YR, et al. Wee1 inhibition by MK1775 potentiates gemcitabine through accumulated replication stress leading to apoptosis in biliary tract cancer. Biomed Pharmacother. 2023,166:115389. doi: 10.1016/j.biopha.2023.115389
    Read this paper
  • Senese S, Cheung K, Lo YC, et al. A unique insertion in STARD9's motor domain regulates its stability. Mol Biol Cell. 2015,26(3):440-52. doi: 10.1091/mbc.E14-03-0829
    Read this paper
  • Cheung AK, Ip JC, Lung HL, et al. Polo-like kinase inhibitor Ro5203280 has potent antitumor activity in nasopharyngeal carcinoma. Mol Cancer Ther. 2013,12(8):1393-401. doi: 10.1158/1535-7163.MCT-12-1219
    Read this paper

Datasheet

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Figure 1. Western blot analysis of WEE1 using anti-WEE1 antibody (A01319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WEE1 antigen affinity purified polyclonal antibody (Catalog # A01319-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WEE1 at approximately 72KD. The expected band size for WEE1 is at 72KD.
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