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Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.

Anti-WNT10A Antibody

HPA013898
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetWNT10A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WNT10A Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80326
  • Target name
    WNT10A
  • Target description
    Wnt family member 10A
  • Target synonyms
    ECTD16, OODD, SSPS, STHAG4, protein Wnt-10a, wingless-type MMTV integration site family, member 10A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZT5
  • Protein Name
    Protein Wnt-10a
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of WNT10A using anti-WNT10A antibody (A03479-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT10A antigen affinity purified polyclonal antibody (Catalog # A03479-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT10A at approximately 46KD. The expected band size for WNT10A is at 46KD.
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