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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA554204(WNT10A Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA554204(WNT10A Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA554204(WNT10A Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

WNT10A Antibody

CSB-PA554204
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetWNT10A
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Overview

  • Supplier
    Cusabio
  • Product Name
    WNT10A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80326
  • Target name
    WNT10A
  • Target description
    Wnt family member 10A
  • Target synonyms
    OODD; protein Wnt-10a; SSPS; STHAG4; wingless-type MMTV integration site family, member 10A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZT5
  • Protein Name
    Protein Wnt-10a
  • Scientific Description
    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitts lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WNT10A using anti-WNT10A antibody (A03479-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT10A antigen affinity purified polyclonal antibody (Catalog # A03479-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT10A at approximately 46KD. The expected band size for WNT10A is at 46KD.
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