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Anti-WNT10A Antibody

107-10957
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetWNT10A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-WNT10A Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Gene ID80326
  • Target name
    WNT10A
  • Target description
    Wnt family member 10A
  • Target synonyms
    ECTD16, OODD, SSPS, STHAG4, protein Wnt-10a, wingless-type MMTV integration site family, member 10A
  • Host
    Rabbit
  • Scientific Description
    WNT10A antibody [N1C1-2], 1 mg/ml (100 ul)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WNT10A using anti-WNT10A antibody (A03479-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT10A antigen affinity purified polyclonal antibody (Catalog # A03479-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT10A at approximately 46KD. The expected band size for WNT10A is at 46KD.
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Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10a antibody [N1C1-2] (GTX111191) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GeneTex
Wnt10a antibody [N1C1-2]GTX111191
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TargetWNT10A
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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA554204(WNT10A Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
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Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
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Anti-WNT10A AntibodyHPA013898
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