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Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-WNT8A Antibody

HPA038539
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetWNT8A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WNT8A Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7478
  • Target name
    WNT8A
  • Target description
    Wnt family member 8A
  • Target synonyms
    WNT8D, protein Wnt-8a, WNT8d, protein Wnt-8d, wingless-type MMTV integration site family, member 8A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1J5
  • Protein Name
    Protein Wnt-8a
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Lane 1: Mouse Heart cell lysates probed with Wnt8A Polyclonal Antibody, Unconjugated (bs-6129R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Wnt8a using anti-Wnt8a antibody (A08392-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: rat C6 whole cell lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt8a antigen affinity purified polyclonal antibody (Catalog # A08392-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt8a at approximately 60KD. The expected band size for Wnt8a is at 60KD.
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IHC image of CSB-PA863951LA01HU diluted at 1:200 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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