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ProductsBack/WNT8A Antibody (aa23-358)

WNT8A Antibody (aa23-358)

LS-C371067
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetWNT8A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    WNT8A Antibody (aa23-358)
  • Delivery Days Customer
    23
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7478
  • Target name
    WNT8A
  • Target description
    Wnt family member 8A
  • Target synonyms
    WNT8D, protein Wnt-8a, WNT8d, protein Wnt-8d, wingless-type MMTV integration site family, member 8A
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Wnt8a using anti-Wnt8a antibody (A08392-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: rat C6 whole cell lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt8a antigen affinity purified polyclonal antibody (Catalog # A08392-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt8a at approximately 60KD. The expected band size for Wnt8a is at 60KD.
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